fluorescence microarray scanner—tecan ls400 laser scanner Search Results


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Microarrays in microtiter plates. (a) <t>NanoPrint</t> <t>Microarrayer</t> spotting recombinant domains onto aldehyde-displaying glass substrates. (b) Attachment of microarrays to the bottom of a bottomless 96-well microtiter plate using an intervening silicone gasket. Panel b of this figure is reproduced, with permission, from the publisher37.
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Microarrays in microtiter plates. (a) <t>NanoPrint</t> <t>Microarrayer</t> spotting recombinant domains onto aldehyde-displaying glass substrates. (b) Attachment of microarrays to the bottom of a bottomless 96-well microtiter plate using an intervening silicone gasket. Panel b of this figure is reproduced, with permission, from the publisher37.
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Microarrays in microtiter plates. (a) <t>NanoPrint</t> <t>Microarrayer</t> spotting recombinant domains onto aldehyde-displaying glass substrates. (b) Attachment of microarrays to the bottom of a bottomless 96-well microtiter plate using an intervening silicone gasket. Panel b of this figure is reproduced, with permission, from the publisher37.
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Microarrays in microtiter plates. (a) <t>NanoPrint</t> <t>Microarrayer</t> spotting recombinant domains onto aldehyde-displaying glass substrates. (b) Attachment of microarrays to the bottom of a bottomless 96-well microtiter plate using an intervening silicone gasket. Panel b of this figure is reproduced, with permission, from the publisher37.
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Microarrays in microtiter plates. (a) <t>NanoPrint</t> <t>Microarrayer</t> spotting recombinant domains onto aldehyde-displaying glass substrates. (b) Attachment of microarrays to the bottom of a bottomless 96-well microtiter plate using an intervening silicone gasket. Panel b of this figure is reproduced, with permission, from the publisher37.
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Microarrays in microtiter plates. (a) <t>NanoPrint</t> <t>Microarrayer</t> spotting recombinant domains onto aldehyde-displaying glass substrates. (b) Attachment of microarrays to the bottom of a bottomless 96-well microtiter plate using an intervening silicone gasket. Panel b of this figure is reproduced, with permission, from the publisher37.
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Image Search Results


Microarrays in microtiter plates. (a) NanoPrint Microarrayer spotting recombinant domains onto aldehyde-displaying glass substrates. (b) Attachment of microarrays to the bottom of a bottomless 96-well microtiter plate using an intervening silicone gasket. Panel b of this figure is reproduced, with permission, from the publisher37.

Journal: Nature protocols

Article Title: Quantifying protein-protein interactions in high throughput using protein domain microarrays

doi: 10.1038/nprot.2010.36

Figure Lengend Snippet: Microarrays in microtiter plates. (a) NanoPrint Microarrayer spotting recombinant domains onto aldehyde-displaying glass substrates. (b) Attachment of microarrays to the bottom of a bottomless 96-well microtiter plate using an intervening silicone gasket. Panel b of this figure is reproduced, with permission, from the publisher37.

Article Snippet: Custom-made aldehyde-coated 74.5 mm × 112.5 mm × 1 mm glass substrates (Thermo Fisher Scientific Inc., cat. no. HAR-1101-C60) ProPlate Gaskets (Grace Bio-labs, cat. no. 204971) 96-Well No-Bottom microtiter plates (Greiner Bio-One, cat. no. 655000) 96-well microtiter plates (Greiner Bio-One, cat. no. 650201) 384-well assay plates, black nonbinding, for FP assays (Corning, cat. no. 3575) 384-well microarray plates, for printing protein microarrays (Genetix, cat. no. X7022) Storage Mat III (Costar, cat. no. 3080) Disposable reagent reservoirs, sterile (VWR, cat. no. 82026-350) Deep-well 96-well microtiter plate (Costar, cat. no. 3960) 14 ml Polypropylene Round-Bottom Tube (Becton Dickinson, cat. no. 352059) NanoPrint LM60 Microarrayer (Arrayit Corporation) including cooling block for source plate and destination block designed for 16 microtiter-sized glass plates Silicon Microarray spotting pins (Parallel Synthesis Technologies Inc., cat. no. SMT-S75) 48-pin Silicon printhead assembly (Parallel Synthesis Technologies Inc., cat. no. SMT-H192) Funnel for silicon 48-pin printhead (Parallel Synthesis Technologies Inc., cat. no. SMT-F48) Fluorescence microarray scanner—Tecan LS400 Laser Scanner or similar (Tecan, cat. no. LS400) ArrayPro software or equivalent (Tecan) Matlab software or equivalent (The MathWorks) Peptide Synthesizer, Apex 396 or similar (Aapptec, cat. no. Apex 396-DC-FW-M) Kromasil 100 (5 μm) C18 semi-prep column (Peeke Scientific, cat. no. 100-5-C18 20 × 250) Kromasil 100 (5 μm) C18 analytical column (Peeke Scientific, cat. no. 100-5-C18 2.1 × 150) Superdex 200 10/300 GL column (Amersham Biosciences, cat. no 17-5175-01) Fluorescence polarization plate reader, Analyst AD 96:384 or similar (LJL Biosystems) Storage Mat Applicator (Corning, cat. no. 3081) Microtiter plate shaker (Lab Line, cat. no. 4625) Floor centrifuge, capable of holding both 500 and 30 ml tubes 2-L Baffled flasks (VWR , cat. no. 89083-696) 500-ml Centrifuge tubes (Sorvall, cat. no. 7-9957) 30-ml Oakridge centrifuge tubes (Thermo Fisher Scientific Inc., cat. no. 3119-0010) P1000 multichannel pipette (Rainin, cat. no. L1000) P200 multichannel pipette (Rainin, cat. no. L200) P10 multichannel pipette (Rainin, cat. no. L10) Mini Bunsen burner Agilent 1200 series HPLC with fraction collector

Techniques: Recombinant